Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Storage Conditions
Vapor phase of liquid nitrogen
Comments
This virus produces foci of round cells on MiCL1(Mink S+L-) cell line, and plaques on PG-4 (feline S+L- cell line). This holding consists of persistently infected tk(-)NIH3T3 producer cells that shed the Mo/A-MLV virus.
Amphotropic
Effect on Host
No CPE in NIH3T3 cells
Recommended Host
Recommended Host: This virus is continuously produced by the cell line in which it is shipped. No additional host is required; however, if desired the virus may be used to infect other cells, which are listed as alternate hosts.
Alternate Host(s): Many mammalian cell lines (murine, rat, human except certain bovine and hamster cells)
Growth Conditions
Incubation: 2 to 7 days at 37°C, a 5% CO2 in air atmosphere is recommended
Name of Depositor
CA Wilson
Source
Transfection of tk(-)NIH3T3 cells with molecular clone of MLV derived by A. Dusty Miller
References
Miller AD, et al. Generation of helper-free amphotropic retroviurses that transduce a dominant-acting, methotrexate-resistant dihydrofolate reductase gene. Mol. Cell. Biol. 5: 431-437, 1985. PubMed: 2985952
General Chapters: <1046> Cell and Gene Therapy Products - Analytical Methodologies. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011
