Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 2.3; BamHI--4.9; BglI--3.4, 1.2, 0.4; PstI--4.4, 0.6; PvuII--2.3, 1.9, 0.6. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--1.51; BglI--1.99; BglII--1.10; ClaI--0.73; EcoRV--0.90; NdeI--0.57, 1.87; NotI--0.06; PstI--0.56; PvuII--1.70; XbaI--1.96; XhoI--0.49. Contains the complete coding sequence (nt 24-1802). Detects transcripts of 2.2 kb and 4.2 kb in the mouse brain. Alternate transcripts of 2.5 kb and 4.4 kb can be detected in other tissues. |