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pHP11 [UTR-9853]

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價格:

編號:B181271

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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產品名稱

pHP11 [UTR-9853]

商品貨號

B181271

Designations

pHP11 [UTR-9853]

GenBank Number

M81457

培養基

Species

Homo sapiens, human

Depositors

TP Dooley

Vector

Construct size (kb): 3.599999904632568

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-7Zf(+)
Intact vector size: 3.000
Type of vector: phagemid
Vector end: EcoRI
Vector end: HindIII
Cloning sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI AsuII ClaII
HindIII BamHI SacI BstXI NsiI
Polylinker sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI AsuII ClaII
HindIII BamHI SacI BstXI NsiI
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1, f1
terminator: none
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CAL1L
Gene name: calpactin I, light polypeptide (p11)
Contains complete coding sequence?: Y
Chromosome: 1; Localization: 1, cR
Tissue: foreskin keratinocyte
Type of DNA: cDNA
Insert 5' end: EcoRI
Insert 3' end: HindIII
Insert size (kb): 0.55
Cross references: DNA Seq. Acc.: M81457
Nucleotides 1-545 of the insert correspond to
nucleotides 1-545 of M81457.

Insert lengths(kb): 0.550000011920929

Tissue: foreskin keratinocyte

Gene product: calpactin I, light polypeptide (p11) [CAL1L]

Insert Size (kb)

0.550

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.9, 0.6; ScaI--2.2, 1.5; NcoI--3.6; XmnI--1.9, 1.5; BamHI--3.5.

The insert contains the following restriction sites (approximate kb from the 5' end): NcoI--0.1; ScaI--0.17; SphI--0.32; XmnI--0.46.

Corresponds to nt 1-545 of the sequence record, including the complete coding region (nt 72-365). The 5' end is linker derived; the 3' end is a naturally occurring site.

References

Dooley TP, et al. cDNA sequence of human p11 calpactin I light chain. Genomics 13: 866-868, 1992. PubMed: 1386341

Thomas P Dooley, personal communication

B242737：pHP11 [UTR-9853]

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