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當前位置: 首頁 > ATCC代理 > Influenza A virus (H1N1) B181285
Influenza A virus (H1N1)
Influenza A virus (H1N1)
規格:
價格:詢價
編號:B181285
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Influenza A virus (H1N1)
商品貨號
B181285
Classification
Orthomyxoviridae, Influenzavirus A
Deposited As
Influenza A virus
培養基
Agent
Influenza A virus (H1N1)
Strain
A/WS/33
Applications
Influenza Research
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Images
VR-1520
Comments
Derived by adaptation of egg allantoic fluid product ATCC® VR-825™ to MDCK cells (ATCC® CCL-34™). ATCC® VR-825™ and ATCC® VR-1520™ have not been compared for sequence or infectivity in eggs and tissue culture. Demonstrates cross-reactivity with Influenza A virus, A/PR/8/34 and swine influenza.
Effect on Host
CPE, cell rounding and sloughing
Recommended Host
MDCK (ATCC® CCL-34™)
Growth Conditions
Temperature: 33°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-4 days
Name of Depositor
WA Chappell, Centers for Disease Control and Prevention; ATCC
Special Collection
NCRR Contract
Source
Throat washings from an influenza patient
References

Burnet FM. A genetic approach to variation in influenza viruses; the characters of three substrains of influenza virus A (WS). J. Gen. Microbiol. 5: 46-53, 1951. PubMed: 14824469

Smith W, et al. A virus isolated from influenza patients. Lancet 2: 66-68, 1933.

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