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pCMV-Cip1
pCMV-Cip1
規格:
貨期:
編號:B181438
品牌:Mingzhoubio

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產品名稱 pCMV-Cip1
商品貨號 B181438
Designations pCMV-Cip1
GenBank Number

L25610

Species Homo sapiens, human
Depositors SJ Elledge
Applications
in another host, produces protein cyclin-dependent kinase inhibitor 1A (p21, Cip1) melanoma differentiation associated protein 6, cyclin-dependent kinase inhibitor (p21), wild type p53 activated fragment 1 (Cdk-interacting protein)
Vector
Construct size (kb): 5.139999866485596
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSXV
Intact vector size: 4.200
Type of vector: plasmid
Vector end: XhoI
Vector end: BamHI
Cloning sites: XhoI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: CMV
replicon: pMB1
terminator: tat
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CDKN1A
Other gene symbols: CIP1; WAF1*
Gene name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Contains complete coding sequence?: Y
Chromosome: 6; Localization: 6 p21.2
Tissue: B-lymphocyte (EBV-transformed)
Type of DNA: cDNA
Insert 5' end: XhoI
Insert 3' end: BglII
Insert size (kb): 1.64
Cross references: DNA Seq. Acc.: L25610
Insert lengths(kb): 1.639999985694885
Tissue: B lymphocyte (EBV transformed)
Gene product: cyclin-dependent kinase inhibitor 1A (p21, Cip1)( melanoma differentiation associated protein 6, cyclin-dependent kinase inhibitor (p21), wild type p53 activated fragment 1 (Cdk-interacting protein)) [CIP1]
Target Gene: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Insert Size (kb) 1.640
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BglI--1.6, 1.1, 0.6, 0.5 (doublet), 0.3 (doublet), 0.2; EcoRI--4.4, 0.9; KpnI--4.8, 0.25; PvuII--4.0, 1.2; XhoI--5.2;
XbaI/NdeI--2.7, 2.0, 0.45; EcoRI/XhoI--3.3, 1.05, 0.9.
Digestion with EcoRI+XhoI yields a 1 kb fragment that can be used as a probe.
Contains the complete coding sequence (nt 79-573 of the sequence record).
When transfected into human cells, expresses the protein from a CMV promoter.
References

Harper JW, et al. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75: 805-816, 1993. PubMed: 8242751

Stephen J Elledge, personal communication

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