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pHRV-mCD40L#3

規格:

價格:詢價

編號:B181884

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

斜面

甘油

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產品名稱

pHRV-mCD40L#3

商品貨號

B181884

Designations

pHRV-mCD40L#3

Depositors

Immunex Corporation, PA Perkins, MK Spriggs, Immunex Corporation

培養基

U.S. Patent

5,565,321

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Biosafety Level

Vector Information

Vector: pHRV-mCD40L#3 (phagemid)

Promoters: Promoter for in vitro transcription PGK-1

Marker(s):ampR,neoR,HSV TK

Construct size (kb): 20.29999923706055

Features: marker(s): HSV TK
marker(s): ampR
marker(s): neoR
promoter for in vitro transcription: PGK-1
replicon: pMB1
MCS: HindIII...SfiI
coding sequence: mouse CD40L

Applications

contains sequences that integrate by homologous recombination CD40 antigen ligand

vector for constructing knockouts CD40 antigen ligand

Comments

Gene targeting vector for CD40 ligand (CD40L, CD154), useful for preparing CD40L-deficient (knockout) mice. Homologous recombination with the vector results in the removal of exons 3 and 4 from the CD40L gene.

The vector can be electroporated into embryonic stem cells, for example at 200 V and 960 uF. Culture the cells on a feeder layer, and select in 175 ug/ml G418+2 mM gancyclovir, for about 10 days. Then plate single clones and analyze via PCR.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

References

Spriggs MK, et al. Detection of mutations in a CD40 ligand gene. US Patent 5,565,321 dated Oct 15 1996

Shipped

frozen

Shipping Information

Distributed: freeze-dried

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