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pES1
pES1
規格:
價格:詢價
編號:B181904
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pES1
商品貨號
B181904
Designations
pES1
Species
Bacillus thuringiensis subsp. kurstaki de Barjac and Lemille
培養基
Depositors
The University of Washington
Applications
produces protein crystal protein (delta endotoxin)
Vector
Construct size (kb): 17.29998970031738
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Bacillus thuringiensis subsp. kurstaki
Gene name: crystal protein (delta endotoxin)
Contains complete coding sequence?: U
Strain: HD-1
Type of DNA: genomic
Insert end: Sau3AI
Insert end: Sau3AI
Insert size (kb): 13.0
Cross references:
DESCRIPTION OF INSERT COMPONENT:
Genome: Bacillus thuringiensis subsp. kurstaki
Gene name: crystal protein (delta endotoxin)
Contains complete coding sequence?: U
Strain: HD-1
Type of DNA: genomic
Insert end: Sau3AI
Insert end: Sau3AI
Insert size (kb): 13.0
Cross references:
Insert lengths(kb): 13.0
Gene product: crystal protein (delta endotoxin)
Target Gene: crystal protein (delta endotoxin)
Insert Size (kb)
13.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Expresses protein recognized by antibodies specific to crystal protein.
The insert contains sequences from the 30, 32, and 47 megadalton plasmids of Bacillus thuringiensis.
References

Schnepf HE, Whiteley HR. Cloning and expression of the Bacillus thuringiensis crystal protein. Proc. Natl. Acad. Sci. USA 78: 2893-2897, 1981. PubMed: 7019914

Schmepf HE, Whiteley HR. Bacillus thuringiensis crystal protein in Escherichia coli. US Patent 4,467,036 dated Aug 21 1984

Schanegl HE, Whiteley HR. Bacillus thuringiensis crystal protein in Escherichia coli. US Patent 4,448,885 dated May 15 1984

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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