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pMCOA12
pMCOA12
規格:
價格:詢價
編號:B181977
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pMCOA12
商品貨號
B181977
Designations
pMCOA12
Species
Homo sapiens, human
培養基
Depositors
RL White, Y Nakamura
Applications
Codominant segregation of the MspI polymorphism has been observed in 19 3-generation families.
TaqI also reveals a polymorphism, but this has not been confirmed by pedigree studies.
Otherwise, RFLPs are observed under normal hybridization and wash stringencies.
Vector
Construct size (kb): 5.699999809265137
Insert
DNA: genomic
Insert lengths(kb): 3.0
Gene product: DNA Segment, single copy [D9S28]
Alleles: A1, A2
Insert Size (kb)
3.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.7; EcoRI/HindIII--3.0, 2.7; EcoRI/BamHI--5.7; BamHI--5.7; PstI--5.7; AccI--3.7, 1.45, + more; consistent with deposited DNA.
Codominant segregation of the MspI polymorphism has been observed in 19 3-generation families.
TaqI also reveals a polymorphism, but this has not been confirmed by pedigree studies.
The probe should be pre-hybridized with excess human DNA before hybridization. Otherwise, RFLPs are observed under normal hybridization and wash stringencies.
Enzyme(s) not detecting polymorphism: BglII, PstI, PvuII, RsaI.
References

Nakamura Y, et al. New approach for isolation of VNTR markers. Am. J. Hum. Genet. 43: 854-859, 1988. PubMed: 2904220

Carlson M, et al. Isolation and mapping of a polymorphic DNA sequence (pMCOA12) on chromosome 9q (D9S28). Nucleic Acids Res. 16: 4744, 1988. PubMed: 2454460

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