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CTLA4Ig

規格:

價格:詢價

編號:B182037

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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產品名稱

CTLA4Ig

商品貨號

B182037

Designations

CTLA4Ig

GenBank Number

I13253

M27286

M27287

培養基

Species

Homo sapiens, human

Depositors

Bristol-Myers Squibb Co., P Linsley, Bristol-Myers Squibb Co.

Applications

in another host, produces protein cytotoxic T-lymphocyte-associated protein 4 - IgCgamma1 chimera

Vector

Construct size (kb): 5.199999809265137

Insert

DNA: cDNA

Insert lengths(kb): 0.4600000083446503

Tissue: HTLV II-associated leukemia cell line H38

Gene product: cytotoxic T-lymphocyte-associated protein 4(cytotoxic T-lymphocyte-associated serine esterase 4) [CTLA4]

Alleles: A1, A2, A3, A4, A5, A6, A7, A8, A9, A10, A11, A12, A13, A14, A15, A16, A17

Target Gene: cytotoxic T-lymphocyte-associated protein 4 - IgCgamma1 chimera

Insert Size (kb)

0.460

Media

ATCC^® Medium 1890: LB medium (ATCC medium 1065) with 12 mcg/ml tetracycline and 100 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): HindIII/XbaI--4.0, 1.2; HindIII--5.2; XbaI--5.2.

Restriction digests of the clone give the following sizes (kb): HindIII--5.2; HindIII/XbaI--4.0, 1.2; PstI--5.2; XbaI--5.2; XhoI--5.2.

The Ig hinge cysteines were mutated to serine residues during construction to prevent intrachain disulfide bonding.

Expresses a soluble derivative of CTLA4, consisting of the oncostatin M signal sequence and partial exon 2 sequence, the extracellular portion of CTLA4, and the hinge, CH2 and CH3 portions of Ig Cgamma1.

A stable CHO transfectant expressing this construct is available as CRL-10762.

References

Linsley PS. Chimeric CTLA4 receptor and methods for its use. US Patent 5,434,131 dated Jul 18 1995

Linsley PS, et al. Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin 2 mRNA accumulation. J. Exp. Med. 173: 721-730, 1991. PubMed: 1847722

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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