| Insert |
DNA: cDNA Insert lengths(kb): 0.4600000083446503 Tissue: HTLV II-associated leukemia cell line H38 Gene product: cytotoxic T-lymphocyte-associated protein 4(cytotoxic T-lymphocyte-associated serine esterase 4) [CTLA4] Alleles: A1, A2, A3, A4, A5, A6, A7, A8, A9, A10, A11, A12, A13, A14, A15, A16, A17 Target Gene: cytotoxic T-lymphocyte-associated protein 4 - IgCgamma1 chimera |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII/XbaI--4.0, 1.2; HindIII--5.2; XbaI--5.2. Restriction digests of the clone give the following sizes (kb): HindIII--5.2; HindIII/XbaI--4.0, 1.2; PstI--5.2; XbaI--5.2; XhoI--5.2. The Ig hinge cysteines were mutated to serine residues during construction to prevent intrachain disulfide bonding. Expresses a soluble derivative of CTLA4, consisting of the oncostatin M signal sequence and partial exon 2 sequence, the extracellular portion of CTLA4, and the hinge, CH2 and CH3 portions of Ig Cgamma1. A stable CHO transfectant expressing this construct is available as CRL-10762. |
| References |
Linsley PS. Chimeric CTLA4 receptor and methods for its use. US Patent 5,434,131 dated Jul 18 1995
Linsley PS, et al. Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin 2 mRNA accumulation. J. Exp. Med. 173: 721-730, 1991. PubMed: 1847722
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