| 產品名稱 | KIM185 |
|---|---|
| 商品貨號 | B182216 |
| Organism | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type | hybridoma:lymphoblast B lymphocyte; somatic cell hybri |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | KIM185 stimulates cell homotypic aggregation by a CD11 pathway in the JY human B lymphoblastoid cell line, and it induces the adherence of neutrophils to protein-coated plastic by a CD11b-dependent mechanism [PubMed: 7690325]. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | Animals were immunized with CR3 (CD11b/CD18). Spleen cells were fused with Sp2/0 myeloma cells. KIM185 stimulates cell homotypic aggregation by a CD11 pathway in the JY human B lymphoblastoid cell line, and it induces the adherence of neutrophils to protein-coated plastic by a CD11b-dependent mechanism [PubMed: 7690325]. The antibody recognizes an epitope distinct from the KIM127 (ATCC CRL-2838) antibody. |
| Comments | Animals were immunized with CR3 (CD11b/CD18). Spleen cells were fused with Sp2/0 myeloma cells. KIM185 stimulates cell homotypic aggregation by a CD11 pathway in the JY human B lymphoblastoid cell line, and it induces the adherence of neutrophils to protein-coated plastic by a CD11b-dependent mechanism [PubMed: 7690325]. The antibody recognizes an epitope distinct from the KIM127 (ATCC CRL-2838) antibody. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing | Protocol: Shake off attached cells and scrape if necessary.
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(4) to 1 X 10(5) viable cells/ml. Interval: Maintain cultures at a cell concentration between 2.5 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation | Freeze medium: Complete growth medium with 20% fetal bovine serum and 7.5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype | mouse IgG1 kappa |
| Population Doubling Time | 20 hours |
| Name of Depositor | MK Robinson |
| Year of Origin | 1989 |
| References | Andrew D, et al. KIM185, a monoclonal antibody to CD18 which induces a change in the conformation of CD18 and promotes both LFA-1- and CR3-dependent adhesion. Eur. J. Immunol. 23: 2217-2222, 1993. PubMed: 7690325 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
