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KIM185
KIM185
規格:
價格:詢價
編號:B182216
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
KIM185
商品貨號
B182216
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma:lymphoblast B lymphocyte; somatic cell hybri
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
KIM185 stimulates cell homotypic aggregation by a CD11 pathway in the JY human B lymphoblastoid cell line, and it induces the adherence of neutrophils to protein-coated plastic by a CD11b-dependent mechanism [PubMed: 7690325].
Storage Conditions
liquid nitrogen vapor phase
Images
Derivation
Animals were immunized with CR3 (CD11b/CD18). Spleen cells were fused with Sp2/0 myeloma cells. KIM185 stimulates cell homotypic aggregation by a CD11 pathway in the JY human B lymphoblastoid cell line, and it induces the adherence of neutrophils to protein-coated plastic by a CD11b-dependent mechanism [PubMed: 7690325]. The antibody recognizes an epitope distinct from the KIM127 (ATCC CRL-2838) antibody.
Comments
Animals were immunized with CR3 (CD11b/CD18). Spleen cells were fused with Sp2/0 myeloma cells. KIM185 stimulates cell homotypic aggregation by a CD11 pathway in the JY human B lymphoblastoid cell line, and it induces the adherence of neutrophils to protein-coated plastic by a CD11b-dependent mechanism [PubMed: 7690325]. The antibody recognizes an epitope distinct from the KIM127 (ATCC CRL-2838) antibody.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Protocol: Shake off attached cells and scrape if necessary. Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(4) to 1 X 10(5) viable cells/ml.
Interval: Maintain cultures at a cell concentration between 2.5 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium with 20% fetal bovine serum and 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
mouse IgG1 kappa
Population Doubling Time
20 hours
Name of Depositor
MK Robinson
Year of Origin
1989
References

Andrew D, et al. KIM185, a monoclonal antibody to CD18 which induces a change in the conformation of CD18 and promotes both LFA-1- and CR3-dependent adhesion. Eur. J. Immunol. 23: 2217-2222, 1993. PubMed: 7690325

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