Restriction digests of the clone give the following sizes (kb): EcoRI--5.3; BamHI--5.3; PstI--3.5, 1.75. The unique Bsp1286I is located within the ori(P). Shuttle vector that can be mobilized by conjugation between E. coli and Pasteurella haemolytica. Constructed by ligating a 979 bp ApaLI fragment from pIC20H (which contains lacZalpha, MCS, and lacI'PO) to ApaLI-digested pPH843. |
