Construction: SV40, Tn5, pBR322, early region of polyoma virus
Marker(s):G418R,ampR
Construct size (kb): 9.2
Features: marker(s): ampR, G418R promoter: SV40 early replicon: pMB1
Applications
expression vector
shuttle vector
Comments
Provides dominant selectable marker for resistance to antibiotic G418 in mammalian cell lines.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Southern PJ, Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Appl. Genet. 1: 327-341, 1982. PubMed: 6286831
