| 產品名稱 |
Rat cytomegalovirus |
| 商品貨號 |
B182273 |
| Classification |
Herpesviridae, Muromegalovirus |
| Agent |
Rat cytomegalovirus |
| Strain |
Priscott |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
tissue, animal Australia Isolation date: 1979 |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Comments |
High concentrations of virus in liver and spleen 14 days after i.c. inoculation. |
| Effect on Host |
Yes, in vitro effects: cytopathic effects (focal rounding) in tissue culture CPE, refractile rounding in TC; death of newborn rats, often with ataxia. Yes, in vivo effects: death of newborn rats, often with ataxia |
| Growth Medium |
Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
|
| Recommended Host |
Recommended Host:? Rat2 (ATCC CRL-1764) |
| Growth Conditions |
Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C Duration: 8-12 days; For best results cells should be 24 to 48 hours old and 70-90% confluent (not 100% confluent). |
| Name of Depositor |
PK Priscott |
| Source |
tissue, animal Australia Isolation date: 1979 |
| Year of Origin |
1979 |
| References |
Priscott PK, Tyrrell DA. The isolation and partial characterisation of a cytomegalovirus from the brown rat, Rattus norvegicus. Arch. Virol. 73: 145-160, 1982. PubMed: 6293409
newborn
wild brown rat [Rattus norvegicus], sub-maxillary gland
|
