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pspT19WDV1
pspT19WDV1
規格:
價格:詢價
編號:B182479
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pspT19WDV1
商品貨號
B182479
Designations
pspT19WDV1
Species
wheat dwarf geminivirus
培養基
Depositors
PM Mullineaux
Vector
Construct size (kb): 4.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 1.0900000333786
Gene product: ORF 1, 10 kDa
Insert Size (kb)
1.090
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): SalI/BamHI--3.2, 1.1; BamHI--4.1.
A NlaIII fragment extending from nt 213 to nt 1303.
The NlaIII fragment was cloned into the SmaI site of pUC18. The SstI-(T4 polI)-BamHI fragment was isolated and inserted into pspT19.
The plasmid can be linearized with SalI for SP6 transcription in vitro.
Classification
Geminivirus
References

Woolston CJ, et al. Agroinfection and nucleotide sequence of cloned wheat dwarf virus DNA. Plant Mol. Biol. 11: 35-43, 1988.

P M Mullineaux, personal communication

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