| 產品名稱 |
pGH101 |
| 商品貨號 |
B182644 |
| Designations |
pGH101 |
| Species |
Escherichia coli (Migula) Castellani and Chalmers |
| Depositors |
GE Herman |
| Vector |
Construct size (kb): 7.599999904632568 |
| Insert |
DNA: genomic Insert lengths(kb): 3.200000047683716 Gene product: beta-galactosidase(beta-D-galactosidase) [lacZ] |
| Insert Size (kb) |
3.200 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
The lacZ cassette can be excised with either BamHI or SalI. This plasmid is suitable for the study of eukaryotic expression. It contains a functional lacZ gene without any regulatory elements. The lacZ gene is a BamHI fragment from pMC1871 and is lacking 8 non-essential N-terminal amino acids. Linkers provide an inframe ATG and new unique BamHI and SalI sites on both ends of the lacZ fragment. |
| Classification |
Enterobacteriaceae, Escherichia |
| References |
Herman GE, et al. An E. coli beta-galactosidase cassette suitable for study of eukaryotic expression. Nucleic Acids Res. 14: 7130, 1986. PubMed: 3093988
Casadaban MJ, et al. Beta-galactosidase gene fusions for analyzing gene expression in Escherichia coli and yeast. Methods Enzymol. 100: 293-308, 1983. PubMed: 6312261
|
