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VHL cosmid 11 in <em>Escherichia coli</em>
VHL cosmid 11 in <em>Escherichia coli</em>
規格:
價格:
編號:B182670
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
VHL cosmid 11 in Escherichia coli
商品貨號
B182670
Designations
VHL cosmid 11 in Escherichia coli
GenBank Definition

Homo sapiens von Hippel-Lindau tumor suppressor (VHL) gene, complete cds

培養基
GenBank Number

AF010238

Depositors
I Kuzmin, National Cancer Institute
Vector
Construct Information
Total size of construct (kb): 48.5
Marker: ampR

Vector Information
Vector: pWek
Vector type: cosmid
Vector size (kb): 8.5
Markers: ampR
Insert
Tissue: renal cell carcinoma
Disease: von Hippel-Lindau tumor
Type of DNA: genomic
Insert ends: BamHI, Sau3AI
Insert size (kb): 40.0
Genbank accession: AF010238 (partial sequence)
Insert Size (kb)
40.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Frozen glycerol stock of E. coli containing the cosmid.
Comments
Contains exon 1 of VHL disease tumor suppressor gene critical region deleted in renal cell carcinomas and approx. 30kb region upstream.
References

Latif F, et al. Identification of the von Hippel-Lindau disease Tumor Suppressor Gene. Science 260: 1317-1320, 1993. PubMed: 8493574

Kuzmin I, et al. One-megabase yeast artificial chromosome and 400-kilobase cosmid-phage contigs containing the von Hippel-Lindau tumor suppressor and Ca(2+)-transporting adenosine triphosphatase isoform 2 genes. Cancer Res. 54: 2486-2491, 1994. PubMed: 8162598

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