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pBY6

規格:

價格:詢價

編號:B182748

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

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產品名稱

pBY6

商品貨號

B182748

Designations

pBY6

Species

Bean yellow mosaic virus

培養基

Depositors

J Hammond

Applications

There was no polyadenylation signal observed.

Vector

Construct size (kb): 3.339999914169312

Insert

DNA: cDNA

Insert lengths(kb): 0.6399999856948853

Gene product: coat protein

Insert Size (kb)

0.640

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

The insert contains a DdeI site and a RsaI site.

Restriction digests of the clone give the following sizes (kb): PstI--2.7, 0.64; EcoRI--3.3; HindIII--3.3.

This construct does not make a fusion protein recognized by MAb PTY1.

The insert includes coding sequences for the carboxy terminal portion of the coat protein gene, 3' untranslated sequences (166 nucleotides) and poly(A) (19 nucleotides).

The 3' non-coding region is A + T rich (61%) and shorter than previously reported potyvirus sequences. There was no polyadenylation signal observed.

Classification

Potyvirus

References

Hammond J, Hammond RW. Molecular cloning, sequencing, and expression in Escherichia coli of the bean yellow mosaic virus coat protein gene. J. Gen. Virol. 70: 1961-1974, 1989. PubMed: 2671258

John Hammond, personal communication

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