| Vector |
Construct size (kb): 3.802999973297119 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752 |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MMP2
Other gene symbols: CLG4A; CLG4
Gene name: matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase,
72kD type IV collagenase)
Contains complete coding sequence?: U
Chromosome: 16; Localization: 16 q12.2
Tissue: placenta
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.117
Cross references: DNA Seq. Acc.: X58968 Insert lengths(kb): 1.116999983787537 Tissue: placenta Gene product: matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase)(collagenase IVA (basement membrane), metalloproteinase stromelysin-2) [CLG4] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.15; BamHI--3.85; HindIII--3.85; PvuII--2.35, 1.2, 0.3; PstI--3.85. The insert contains the following restriction sites (bp from the 5' end): AvaI--204, 253; HaeIII--75, 111, 180, 221, 252, 558, 757, 889, 988; MboI--497, 604, 616, 635, 745; PvuII--973. Contains 236 bp of the 5' untranslated region (starting 50 bp downstream of the transcription start point) and encodes the amino terminal portion of the protein. Detects a transcript of 3.1 kb. |
| References |
Levy AT, et al. Increased expression of the Mr 72,000 type IV collagenase in human colonic adenocarcinoma. Cancer Res. 51: 439-444, 1991. PubMed: 1846313
William Stetler-Stevenson, personal communication
|
