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pH3a
pH3a
規格:
價格:詢價
編號:B182785
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pH3a
商品貨號
B182785
Designations
pH3a
GenBank Number

X58968

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 3.802999973297119
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MMP2
Other gene symbols: CLG4A; CLG4
Gene name: matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase,
72kD type IV collagenase)
Contains complete coding sequence?: U
Chromosome: 16; Localization: 16 q12.2
Tissue: placenta
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.117
Cross references: DNA Seq. Acc.: X58968
Insert lengths(kb): 1.116999983787537
Tissue: placenta
Gene product: matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase)(collagenase IVA (basement membrane), metalloproteinase stromelysin-2) [CLG4]
Insert Size (kb)
1.117
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.15; BamHI--3.85; HindIII--3.85; PvuII--2.35, 1.2, 0.3; PstI--3.85.
The insert contains the following restriction sites (bp from the 5' end): AvaI--204, 253; HaeIII--75, 111, 180, 221, 252, 558, 757, 889, 988; MboI--497, 604, 616, 635, 745; PvuII--973.
Contains 236 bp of the 5' untranslated region (starting 50 bp downstream of the transcription start point) and encodes the amino terminal portion of the protein.
Detects a transcript of 3.1 kb.
References

Levy AT, et al. Increased expression of the Mr 72,000 type IV collagenase in human colonic adenocarcinoma. Cancer Res. 51: 439-444, 1991. PubMed: 1846313

William Stetler-Stevenson, personal communication

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