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pKB132
pKB132
規格:
價格:詢價
編號:B182921
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pKB132
商品貨號
B182921
Designations
pKB132
GenBank Number

M73626

培養基
Species
Sonchus yellow net virus
Depositors
AO Jackson
Vector
Construct size (kb): 0.0
Insert
DNA: cDNA
Insert lengths(kb): 0.7009999752044678
Gene product: glycoprotein (viral envelope) [G]
Insert Size (kb)
0.701
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.2, 0.7; PvuII--2.8, 1.1; NdeI/HindIII--3.2, 0.7.
The insert contains the following restriction sites (nt from the 5' end): NdeI--60; EcoRV--671, 678.
Includes a portion of the coding sequence (corresponding to part of the carboxy terminal region). The complete coding region extends from nt 35-1933 of the published sequence.
Subcloned from pAS3-9.
The 5' end of the insert (which is the 5' portion of the carboxy portion of the ORF) is adjacent to the EcoRI site of the MCS.
Classification
Nucleorhabdovirus
References

Goldberg KB, et al. Structure of the glycoprotein gene of Sonchus yellow net virus, a plant rhabdovirus. Virology 185: 32-38, 1991. PubMed: 1926779

Andrew O Jackson, personal communication

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