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pUC1813-NoriT

規格:

價格:詢價

編號:B182986

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pUC1813-NoriT

商品貨號

B182986

Designations

pUC1813-NoriT

Species

pCU1

培養基

Depositors

PN Hengen

Vector

Construct size (kb): 4.199999809265137

Insert

DNA: genomic

Insert lengths(kb): 1.5

Gene product: (IncN) origin of transfer replication [oriT]

Insert Size (kb)

1.5

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.5; HindIII--2.6, 1.6; BamHI--2.7, 1.5; PstI--2.55, 1.65.

Plasmid containing an oriT cassette bounded by multiple restriction sites for ease in subcloning.

The order of elements in the polylinker is: HindIII PstI XbaI BamHI SmaI KpnI - insert EcoRI HindIII oriT BamHI EcoRI insert - SmaI BamHI XbaI PstI HindIII.

References

Hengen PN, Iyer VN. DNA cassettes containing the origin of transer (oriT) of two broad-host-range transfer systems. BioTechniques 13: 56-62, 1992. PubMed: 1503776

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