Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.5; HindIII--2.6, 1.6; BamHI--2.7, 1.5; PstI--2.55, 1.65. Plasmid containing an oriT cassette bounded by multiple restriction sites for ease in subcloning. The order of elements in the polylinker is: HindIII PstI XbaI BamHI SmaI KpnI - insert EcoRI HindIII oriT BamHI EcoRI insert - SmaI BamHI XbaI PstI HindIII. |
