| 產品名稱 |
Human echovirus 12 |
| 商品貨號 |
B183029 |
| Classification |
Picornaviridae, Enterovirus, Human enterovirus B |
| Deposited As |
Echovirus 12 |
| Agent |
Human echovirus 12 |
| Strain |
Travis 2-85 |
| Common Name |
Human echovirus 12 |
| Positive for Mycoplasma |
No |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
derived from existing strain |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder
(or colder) |
| Effect on Host |
Yes, in vitro effects: Cytopathic effects, refractile rounding and sloughing of cells CPE, refractile rounding and sloughing of cells Yes, in vitro effects: agglutinates human type O red blood cells |
| Growth Medium |
Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
|
| Recommended Host |
Recommended Host:? LLC-MK2 (ATCC CCL-7.1) |
| Growth Conditions |
Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C Duration: 2-5 days; For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent). |
| Cryopreservation |
Storage temperature: -70°C |
| Name of Depositor |
ATCC, HA Wenner, PS Kamitsuka |
| Special Collection |
ATCC |
| Source |
derived from existing strain |
| References |
Hammon WM, et al. Problems raised by certain ECHO viruses in the attempted laboratory detection of poliomyelitis virus infection. Ann. N.Y. Acad. Sci. 67: 304-310, 1957. PubMed: 13411968
or colder
VR-1563 was created by solvent treating Mycoplasma orale positive VR-42 [which was isolated in 1952 from a rectal swab taken from a 6-year-old healthy American male, Philippine Islands] and passaging it three times and finding no detectable Mycoplasma by PCR.
This holding has no detectable mycoplasma by PCR.
or colder
|
