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當(dāng)前位置: 首頁 > ATCC代理 > HCL-1 B183041
HCL-1
HCL-1
規(guī)格:
價格:詢價
編號:B183041
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
HCL-1
商品貨號
B183041
Designations
HCL-1
Species
Homo sapiens, human
培養(yǎng)基
Depositors
Y Israel
Vector
Construct size (kb): 4.099999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK-
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CTH
Genomic copy number: unique
Gene name: cystathionase (cystathionine gamma-lyase)
Contains complete coding sequence?: U
Chromosome: 16; Localization: 16
Tissue: liver
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.1
Cross references:
Insert lengths(kb): 1.100000023841858
Tissue: liver
Gene product: cystathionine gamma-lyase(cystathionase (cystathionine gamma-lyase)) [CTH]
Insert Size (kb)
1.100
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.1; BglII--4.1; BamHI--4.1; HindIII--4.1; XbaI--4.1.
The insert contains the following restriction sites (bp from the 5' end): BglI--88; BamHI--114; AvaII--128; AvaI--198; ScaI--278; DraI--333; XmnI--589; BstEII--840; BspHI--959; NsiI--968; BglII--1065; XbaI--1068; HindIII--1076.
Contains a complete coding sequence, as a variant missing 132 bp included in other cDNAs derived from liver.
Detects a 2.5 kb mRNA in human liver.
Transcription from the T3 promoter gives the sense strand.
Enzymes not detecting polymorphism: TaqI, XbaI, BglI, RsaI, HindIII, MspI, PstI, EcoRV, ScaI, SacI, BamHI, NcoI, Sau3A, PvuII.
References

Chua AO, et al. Expression cloning of a human IL-12 receptor component--a new member of the cytokine receptor superfamily with strong homology to gp130. J. Immunol. 153: 128-136, 1994. PubMed: 7911493

Lu Y, et al. Cloning and nucleotide sequence of human liver cDNA encoding for cystathionine gamma-lyase. Biochem. Biophys. Res. Commun. 189: 749-758, 1992. PubMed: 1339280

The last 13 nucleotides of the coding sequence are missing.---ATCC staff

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