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pRCgammaA-2
pRCgammaA-2
規格:
價格:詢價
編號:B183138
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pRCgammaA-2
商品貨號
B183138
Designations
pRCgammaA-2
GenBank Number

X52815

培養基
Species
Rattus norvegicus, rat
Depositors
CW Brown
Vector
Construct size (kb): 4.809999942779541
Insert
DNA: cDNA
Insert lengths(kb): 1.845999956130981
Tissue: kidney
Gene product: actin, gamma 1 [ACTG1]
Insert Size (kb)
1.846
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The insert contains the following unique sites in nucleotides from the 5' end: KpnI--931, XbaI--1148, HindIII--1283.
Restriction digests of the clone give the following sizes (kb): BamHI--4.8; HindIII--3.6, 1.2; PstI--3.0, 1.8; EcoRI--4.8; XbaI/XhoI--3.0, 1.1, 0.7.
At the 5' end, the insert includes poly(G)11 and 12 5' untranslated nucleotides. The insert contains a complete open reading frame (nucleotides 23 - 1148), and extends to the PstI site at 1848 just 5' to the poly(A).
The 3' untranslated region contains 2 inverted repeat sequences and a consensus 'TATA' transcription initiation signal.
The insert is oriented such that transcription from the T7 promoter gives the sense strand.
References

Brown CW, et al. A cDNA sequence encoding cytoskeletal gamma-actin from rat. Nucleic Acids Res. 18: 5312, 1990. PubMed: 2402472

Chester W Brown, personal communication

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