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pVE650 [MA6618]
pVE650 [MA6618]
規(guī)格:
貨期:
編號:B183234
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pVE650 [MA6618]
商品貨號 B183234
Designations pVE650 [MA6618]
Species Streptomyces avermitilis (ex Burg et al.) Kim and Goodfellow
Depositors Merck & Co., Inc.
Applications
produces protein
Vector
Construct size (kb): 47.79000091552734
Insert
DNA: genomic
Insert lengths(kb): 24.0
Gene product: [avrB]
Insert Size (kb) 24.0
Media ATCC® Medium 1339: R2 YE medium
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
The plasmid contains the following BamHI fragments (kb): 24.05, 7.4, 4.7, 3.0, 2.35, 2.09, 1.85, 1.4, 1.09, 0.53, 0.02.
Positioned between approximately 60-84 kb on the 110 kb map containing the avermectin gene cluster.
avrC is included in a 4.28 kb BglII fragment and a 4.05 EcoRI fragment; and avrD is included in a 2.56 kb BglII fragment.
The avrB, avrC, and avrD genes map to BglII fragments of 4.3, 0.1, and 2.6 kb at one end of the clone.
References

MacNeil DJ, et al. Analysis of Streptomyces avermitilis genes required for avermectin biosynthesis utilizing a novel integration vector. Gene 111: 61-68, 1992. PubMed: 1547955

Gewain KM, et al. Cloning genes from Streptomyces avermitilis for avermectin biosynthesis and the methods for their use. US Patent 5,252,474 dated Oct 12 1993

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
梅經(jīng)理 17280875617 1438578920
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周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
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李經(jīng)理 13626845108 972239479