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pBH302
pBH302
規格:
價格:詢價
編號:B183255
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pBH302
商品貨號
B183255
Designations
pBH302
Species
Homo sapiens, human
培養基
Depositors
WE Bradley
Vector
Construct size (kb): 5.0
Insert
DNA: cDNA
Insert lengths(kb): 2.0
Tissue: placenta
Gene product: thyroid hormone receptor, beta (avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 2) [THRB]
Alleles: A2, A2, A1, B1, B2, D1, E1, E2, A1, B1, B2, D1, E1, E2, D2, D2
Insert Size (kb)
2.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 2.0; EcoRI--4.9; HindIII--4.9; PstI--4.1, 0.8; EcoRI/PstI--2.9, 1.2, 0.8.
The insert was subcloned from lambdaPGE302 and includes a short exon in the 5' untranslated region of erbAbeta.
This probe contains some low repeat sequences. Good results are obtained by hybridizing in the presence of 25 ug/ml human DNA, although it may not be necessary.
Enzyme(s) not detecting polymorphism: EcoRI, MspI, PstI, TaqI, PvuII, BamHI.
Enzyme(s) not detecting polymorphism: EcoRI, TaqI, PvuII, BamHI, BglII, RsaI, HinfI.
References

Gareau JL, et al. A frequent HindIII RFLP on chromosome 3p21-25 detected by a genomic erbAbeta sequence. Nucleic Acids Res. 16: 1223, 1988. PubMed: 2894009

Usala SJ, et al. Tight linkage between the syndrome of generalized thyroid hormone resistance and the human c-erbA(beta) gene. Mol. Endocrinol. 2: 1217-1220, 1988. PubMed: 2905763

Botto M, et al. EcoRI polymorphism in the human third complement component (C3) gene. Nucleic Acids Res. 18: 2833, 1990. PubMed: 1971096

Hosoe S, et al. Localization of the Von Hippel-Lindau disease gene to a small region of chromosome 3. Genomics 8: 634-640, 1990. PubMed: 2276737

Douglas JB, et al. A pulsed-field gel electrophoresis map locates the polymorphic probes for ERBA2 and ErbAbeta within 120 kb of each other, confirming that THRB (formerly ERBA2) maps to chromosome 3. Genomics 9: 398-400, 1991. PubMed: 1672299

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