| Vector Information |
Size (kb): 4.788
Vector: pRS414 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pBluescriptII KS+
Marker(s):TRP1,ampR
Construct size (kb): 4.788
Features: insert detection: lacZ'
marker(s): TRP1
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARSH4
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
centromere: CEN6 |
| Comments |
Restriction digests of the clone give the following sizes (kb): PvuII--4.35, 0.45; BglI--3.1, 1.7; HindIII--3.85, 0.95. A YC-type shuttle vector with TRP1 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites. |
