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pE194-cop6 [BSGC 1E7]
pE194-cop6 [BSGC 1E7]
規格:
貨期:
編號:B184539
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pE194-cop6 [BSGC 1E7]
商品貨號 B184539
Designations pE194-cop6 [BSGC 1E7]
Depositors CPC International, Inc.
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level 1
Host
Distribution host: Bacillus subtilis BSV IE7
Vector Information
Construct size (kb): 3.599999904632568
Applications
shuttle vector
Comments
For full induction, the plasmid requires a period of induction by subinhibitory concentrations of erythromycin.
Temperature sensitive.
Plasmid is a high copy mutant of pE194, a natural isolate from Staphylococcus aureus that confers resistance to macrolide, lincosamide and streptogramin type B ('MLS') antibiotics.
Media ATCC® Medium 18: Trypticase Soy Agar/Broth
Growth Conditions
Temperature: 30.0°C
References

Dean DH, Dooley MM. Plasmid cloning vector for Bacillus subtilis. US Patent 4,419,450 dated Dec 6 1983

Weisblum B, et al. Plasmid copy number control: isolation and characterization of high-copy-number mutants of plasmid pE194. J. Bacteriol. 137: 635-643, 1979. PubMed: 104975

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