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5HT1Dbeta
5HT1Dbeta
規格:
價格:
編號:B184550
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
5HT1Dbeta
商品貨號
B184550
Designations
5HT1Dbeta
GenBank Number

L09732

培養基
Species
Homo sapiens, human
Applications
in another host, produces protein 5-hydroxytryptamine (serotonin) receptor 1D beta
Vector
Construct size (kb): 8.710000038146973
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: p3CLNeo
Intact vector size: 7.440
Type of vector: plasmid
Vector end: HindIII
Vector end: SalI
Cloning sites: HindIII SalI
Polylinker sites: HindIII SalI
Construction: p3CL, neoR
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, G418
promoter: CMV
replicon: pMB1, SV40
terminator: bGH polyadenylation
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: HTR1DB*
Gene name: 5-hydroxytryptamine (serotonin) receptor 1D beta
Contains complete coding sequence?: Y
Tissue: placenta, male
Type of DNA: genomic
Insert 5' end: HindIII
Insert 3' end: SalI
Insert size (kb): 1.27
Cross references: DNA Seq. Acc.: L09732
Insert lengths(kb): 1.269999980926514
Tissue: placenta, male
Gene product: 5-hydroxytryptamine (serotonin) receptor 1D beta [HTR1DB*]
Target Gene: 5-hydroxytryptamine (serotonin) receptor 1D beta
Insert Size (kb)
1.270
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BglI--3.2, 1.9, 1.8, 1.1, 0.7; HindIII/SalI--7.4, 1.3; PstI--6.5, 1.3, 0.9; PvuII--3.5, 2.3, 1.1, 0.78, 0.58, 0.44; SmaI--5.7, 3.0; EcoRI--6.0, 3.0; EcoRI/XhoI--6.0, 2.35, 0.65.
The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.30; NcoI--0.03; PstI--0.13; PvuII--0.80; SmaI--0.82.
Includes 22 bp of 5' untranslated sequence, the complete coding region, and 64 bp of 3' untranslated sequence.
The order of the major features of the plasmid is: ampR - EcoRI - SV40 ori - SV40 promoter - XhoI - neoR - SV40 polyadenylation - EcoRI - CMV promoter - HindIII - insert - SalI - bGH polyadenylation - PvuI.
References

Veldman SA, Bienkowski MJ. Cloning and pharmacological characterization of a novel human 5-hydroxytryptamine1D receptor subtype. Mol. Pharmacol. 42: 439-444, 1992. PubMed: 1328844

Sarah A Veldman, personal communication

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