The insert contains the following sites separated by (bp)(approx): SmaI- 600- EcoRI- 210- BamHI- 231- PstI. Restriction digests of the clone give the following sizes (kb): PstI--3.7; BglI--2.6, 1.1; HindIII--3.7; EcoRI--3.1, 0.6; BamHI--3.7. Insert is in a reverse orientation in pUC18. |
