| 產(chǎn)品名稱 |
HHCPH74 |
| 商品貨號(hào) |
B184706 |
| Designations |
HHCPH74 |
| GenBank Number |
M79017
|
| Species |
Homo sapiens, human |
| Vector |
Construct size (kb): 5.900000095367432 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: EF1B*
Gene name: elongation factor 1, beta
Contains complete coding sequence?: U
Tissue: hippocampus, female, 2 years, oligo-dT and random primed
(Stratagene 936205), subtracted
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.9
Cross references: DNA Seq. Acc.: M79017; EST: EST01165; Primer: M13 Forward;
THC id: THC122575
DNA Seq. Acc.: X60656; EST: EST01165
Nucleotides 162-1 of the insert correspond to
nucleotides 656-817 of X60656 with 98% identity. Insert lengths(kb): 2.900000095367432 Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted Gene product: elongation factor 1, beta [EF1B*] |
| Insert Size (kb) |
2.900 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.1, 1.8, 1.15; PstI--2.9 (doublet). The insert contains multiple EcoRI fragments. It is important to note these may have been derived from different cDNAs, especially because human EF1beta sequences are less than 1 kb. Based on the alignment with the record for EF1beta in EMBL accession X60656 (818 nt, CDS 96-773), the smaller EcoRI fragment appears to contain most of the cDNA. |
| References |
Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749
J C Venter, personal communication
|
