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HHCPH74
HHCPH74
規格:
價格:
編號:B184706
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HHCPH74
商品貨號
B184706
Designations
HHCPH74
GenBank Number

M79017

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 5.900000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: EF1B*
Gene name: elongation factor 1, beta
Contains complete coding sequence?: U
Tissue: hippocampus, female, 2 years, oligo-dT and random primed
(Stratagene 936205), subtracted
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.9
Cross references: DNA Seq. Acc.: M79017; EST: EST01165; Primer: M13 Forward;
THC id: THC122575
DNA Seq. Acc.: X60656; EST: EST01165
Nucleotides 162-1 of the insert correspond to
nucleotides 656-817 of X60656 with 98% identity.
Insert lengths(kb): 2.900000095367432
Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted
Gene product: elongation factor 1, beta [EF1B*]
Insert Size (kb)
2.900
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.1, 1.8, 1.15; PstI--2.9 (doublet).
The insert contains multiple EcoRI fragments. It is important to note these may have been derived from different cDNAs, especially because human EF1beta sequences are less than 1 kb.
Based on the alignment with the record for EF1beta in EMBL accession X60656 (818 nt, CDS 96-773), the smaller EcoRI fragment appears to contain most of the cDNA.
References

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

J C Venter, personal communication

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