Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Effect on Host
CPE, rounding and sloughing
Recommended Host
BHK-21 [C-13] (ATCC® CCL-10™)
Growth Conditions
Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 30-50% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 30 minutes at 37°C in a humidified 5% CO2 atmosphere, rocking every 10-15 minutes to redistribute inoculum. End adsorption by adding virus growth medium Incubation: 4-5 days
Name of Depositor
SE Grossberg, Medical College of Wisconsin
Chain of Custody
ATCC <-- SE Grossberg, Medical College of Wisconsin
