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HGR3.5 [HUMGRA2]
HGR3.5 [HUMGRA2]
規格:
價格:
編號:B185065
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HGR3.5 [HUMGRA2]
商品貨號
B185065
Designations
HGR3.5 [HUMGRA2]
GenBank Number

M73816

培養基
Species
Homo sapiens, human
Depositors
MV Govindan
Vector
Construct size (kb): 7.900000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GRL
Genomic copy number: unique
Gene name: glucocorticoid receptor
Contains complete coding sequence?: Y
Chromosome: 5; Localization: 5 q31-q32
Tissue: leukocyte
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 3.5
Cross references: DNA Seq. Acc.: M73816
Nucleotides 1-1628 of the insert correspond to
nucleotides 1-1628 of M73816 with 100% identity.
Insert lengths(kb): 3.5
Tissue: leukocyte
Gene product: glucocorticoid receptor [GRL]
Insert Size (kb)
3.5
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BglI--3.3, 2.4, 2.0, 0.26; EcoRI--4.4, 3.4; HindIII--4.8, 1.1, 0.68, 0.60, 0.50, 0.30; PvuII--3.9, 2.4, 1.8; SalI--6.5, 1.6.
The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.93; BglII--0.64, 1.20; HindIII--0.14, 0.77, 1.34; PvuII--0.16; SalI--0.81.
Contains part of intron 1, exon 2 and part of intron 2.
Subcloned from lambdaGHGR-5. ATCC 80008/9 and ATCC 80010/11 are contiguous in the genome.
References

Govindan MV, et al. Human glucocorticoid receptor gene promoter-homologous down regulation. J. Steroid Biochem. Mol. Biol. 40: 317-323, 1991. PubMed: 1958537

Leclerc S, et al. Purification of a human glucocorticoid receptor gene promoter-binding protein. J. Biol. Chem. 266: 8711-8719, 1991. PubMed: 2026589

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