A 2.0 kb BamHI fragment of the insert has been used to detect a mRNA of about 4.3 kb which was present at higher concentration in galactose-grown Saccharomyces.
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
The insert contains the following sites separated by (kb)(approx): SstI- 0.5- HindIII- 0.4- BamHI- 0.9- HindIII- 1.2- BamHI- 1.3- EcoRI- 0.5- EcoRI.
Restriction digests of the clone give the following sizes (kb): SmaI/SalI--7.0 (doublet); SalI--13.7; EcoRV--12.9, 0.8; XbaI--8.0, 5.7.
A 2.0 kb BamHI fragment of the insert has been used to detect a mRNA of about 4.3 kb which was present at higher concentration in galactose-grown Saccharomyces.
A regulatory element has been demonstrated between -354 and -143 (relative to +1 being the A of the ATG initiation signal).
pG70/T1 is available in Escherichia coli RR1 (ATCC 37661) and in Saccharomyces cerevisiae C225/U2 (ATCC 66320).
Repetto B, Tzagoloff A. Structure and regulation of KGD1, the structural gene for yeast alpha-ketoglutarate dehydrogenase. Mol. Cell. Biol. 9: 2695-2705, 1989. PubMed: 2503710
