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<em>Metarhizium brunneum</em> Petch
<em>Metarhizium brunneum</em> Petch
規格:
貨期:
編號:B186462
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 Metarhizium brunneum Petch
商品貨號 B186462
Deposited As Metarhizium anisopliae (Metschnikoff) Sorokin var. anisopliae
Strain Designations M.a. 43 [HRI 275.86]
Application
Biological control of vine weevil, Otiorhynchus sulcatus
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Preceptrol&reg; no
Comments Compatibility with pesticides

This fungus was re-identified as Metarhizium brunneum based on the multigene sequence analysis
Morphology On Blakeslee’s malt extract medium at 25°C after 15 days, two mycelial types were observed. White mycelium, cottony to floccose, dense. Yellowish tan mycelium velutinous to powdery with abundant dark brown to black sporodochium. Reverse creamy white to yellowish tan. Hyphae hyaline, guttulate. Conidia honey brown, elongated ovoid, smooth, single and in chains, 6-8 X 1.5-3 μm.
Medium ATCC® Medium 323: Malt agar medium
ATCC® Medium 325: Malt extract agar (Blakeslee's formula)
ATCC® Medium 336: Potato dextrose agar (PDA)
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence

TCTCCGTTGGTGAACCAGCGGAGGGATCATTACCGAGTTATCCAACTCCCAACCCCTGTGAATTATACCTTTAATTGTTGCTTCGGCGGGACTTCGCGCCCGCCGGGGACCCAAACCTTCTGAATTTTTTAATAAGTATCTTCTGAGTGGTTAAAAAAATGAATCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGTCAGTATTCTGGCGGGCATGCCTGTTCGAGCGTCATTACGCCCCTCAAGTCCCCTGTGGACTTGGTGTTGGGGATCGGCGAGGCTGGTTTTCCAGCGCAGCCGTCCCTCAAATCAATTGGCGGTCTCGCCGTGGCCCTCCTCTGCGCAGTAGTAAAACACTCGCAACAGGAGCCCGGCGCGGTCCACTGCCGTAAAACCCCCCAACTTTTTATAGTTGACCTCGAATCAGGTAGGACTACCCGCTGAACTTAAGCATATCAATAA


D1D2 region of the 28S ribosomal RNA gene

ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGTCCCCAGGGCCCGAGTTGTAATTTGCAGAGGATGCTTTTGGTGAGGTGCCTTCCGAGTTCCCTGGAACGGGACGCCATAGAGGGTGAGAGCCCCGTCTGGTTGGATACCGAGCCTCTGTAAAGCTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCTAAATGGGAGGTATATGTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGGGTTAAATAGTACGTGAAATTGTTGAAAGGGAAGCACTTATGACCAGACTTGGCCCCGGTGAATCATCCAGCGGTTCCCGTTGGTGCACTTTGCCGGGGTTCAGGCCAGCATCAGTTCGCTCCGGGGGATAAAGGCTTTGGGAATGTGGCTCCCTCGGGAGTGTTATAGCCCATTGCGCAATACCCTGTGGCGGGCTGAGGTTCGCGCTTTATGCAAGGATGCTGGCATAATGGTCATCAGTGAC


Elongation factor 1-alpha gene

TCACATCAACGTGGTCGTTATCGTAAGTCGCCTGCCTCCATTTCGAGCCTTGTAGAAGCTGTTATACTGACTTGCTTGTCGTAGGGGTATGTTTCGGAGCCTACACTCTTCGCCGTCTCGAGTTTGTGATAACTGACTGGTCCTCACAGCCACGTCGACTCCGGCAAGTCTACCACCACTGGTCACTTGATCTACCAGTGCGGTGGTATCGACAAGCGTACCATTGAGAAGTTCGAGAAGGTAAGCCAAACCACTCCGATTAATGATCTGCTATTGTTTGGCGATGAACACTATTGGGTTTCCCGCTGTCTGTCGGCCATTACCCCTCACTGTGACACGAAAATTTTCGCGGGGCCTTTATCTTGAACTTTGGTGGGGCACCATACCCCGCCAGCTGTCGAGGGTGTCTCTTTGTGTCTCTGGCTGTTGAAACCACAATATTGTCGTTGCTTTCAGAGGAAAAAACATTAAACTAATTTGGATCGCTGTATAGGAAGCCGCTGAACTCGGCAAGGGTTCCTTCAAGTACGCATGGGTTCTTGACAAGCTCAAGGCCGAGCGTGAGCGTGGTATCACCATCGACATTGCCCTCTGGAAGTTCGAGACTCCCAAGTACTATGTCACCGTCATTGGTATGTCGACTTGCGCCAACTGACCGCATACTGTTCTCCCAAATTGAATGCTAATGCCCCTCCCACAGACGCTCCCGGTCACCGTGACTTTATCAAG


beta tubulin gene

CCCCCCTCCATTGTCTAGGACCCCTCCTCGACGCGTTCCATGGTCGTGCCCCTGATTTTGGTACCCCGCTGTATGAGGAGGCCACGTCCATATACGCACGCCCAACGGATCATCAGGACGAGGCAAAACGCGCTGCATCATCATAATGAACATTGGCTGACGATTTATTTCCTTTTGATCTTACACAGGTTCACCTTCAGACCGGTCAGTGCGTAAGTAAACAAATTCGCCGCTATTTTCGAATGAATTGTTACGGAGACCGGGAGCTTACAATATTTCGACAGGGTAACCAAATTGGTGCTGCTTTCTGGCAGACCATCTCTGGCGAACATGGCCTCGACAGCAATGGTGTCTACAACGGTACCTCTGAGCTCCAGCTCGAGCGTATGAGCGTCTACTTCAATGAGGTAAGTTTGAATGAGCCGAATCCTAGGATGTTGAGGAACCATAGGTTGTTATCCATGGGGAGATCAAAGACTGACGCGGGGACTCCCCTCAAAAGGCCTCCGGTAACAAGTATGTTCCTCGCGCCGTCCTTGTCGATCTTGAACCTGGCACCATGGATGCCGTCCGTGCCGGTCCTTTCGGTCAGCTTTTCCGTCCCGACAACTTCGTCTTTGGTCAGTCTGGTGCTGGCAACAACTGG
Name of Depositor G Zimmermann
Chain of Custody
ATCC <-- G Zimmermann <-- E Muller-Kogler
Isolation
Codling moth larvae, Cydia pomonella, Austria
References

Moorhouse ER, et al. Influence of fungicides and insecticides on the entomogenous fungus Metarhizium anisopliae, a pathogen of the vine weevil, Otiorhynchus sulcatus. Biocontrol Sci. Technol. 2: 49-58, 1992.

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