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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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pBKE
pBKE
規格:
價格:
編號:B186881
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pBKE
商品貨號
B186881
Designations
pBKE
Species
Homo sapiens, human
培養基
Depositors
SB Mizel
Vector
Construct size (kb): 4.03000020980835
Insert
DNA: Synthetic
Insert lengths(kb): 0.0390000008046627
Gene product: immunoglobulin kappa enhancer [IGK*]
Insert Size (kb)
0.039
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.1; EcoRI--4.1; PvuII--4.1; HindIII--uncut; EcoRI/BamHI--4.1.
In 70Z/3 cells, kappa enhancer binding activity was absent in unstimulated cells, but was induced in cells incubated with lipopolysaccharide and interleukin-1, forskolin, or BrcAMP.
The sequence of the insert, from the EcoRI to the BamHI sites is: GAATTCGGCA TCTCAACAGA GGGGACTTTC CGAGAGGCGG ATCC. The string of 4 G's begins the 11 bp NF-kappaB-binding site.
References

Shirakawa F, Mizel SB. In vitro activation and nuclear translocation of NF-kappaB catalyzed by cyclin AMP-dependent protein kinase and protein kinase C. Mol. Cell. Biol. 9: 2424-2430, 1989. PubMed: 2548081

Shirakawa F, et al. Interleukin 1 and cyclic AMP induce kappa immunoglobulin light-chain expression via activation of an NF-kappaB-like DNA-binding protein. Mol. Cell. Biol. 9: 959-964, 1989. PubMed: 2542770

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