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pHER2-436-1 plasmid in <em>E. coli</em>
pHER2-436-1 plasmid in <em>E. coli</em>
規格:
價格:
編號:B187415
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pHER2-436-1 plasmid in E. coli
商品貨號
B187415
Designations
pHER2-436-1 plasmid in E. coli
Depositors
A Ullrich
培養基
Vector
Total size of construct (kb): 4.2
Excise Insert: ExoRI or SalI
Vector: pUC12
Vector type: plasmid
Vector size (kb): 2.7
Markers: ampR
Insert
Insert size (kb): 1.45
Gene: Avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog, ERBB2
DNA: cDNA
Source: Human
Insert Size (kb)
1.450
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Freeze dried E. coli containing the plasmid
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.5; BamHI--4.1, 0.4; HindIII--4.3; PstI--3.1, 1.0, +2 smaller; SalI--2.7, 1.5; SacI--2.65, 1.5.
This is the 5' EcoRI fragment of lambdaHER2-436 which begins about 150 bp upstream of the initiation codon and encodes the first 433 amino acids--including the signal sequence and 4 potential glycosylation sites.
References

Coussens L, et al. Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene. Science 230: 1132-1139, 1985. PubMed: 2999974

Tal M, et al. Human HER2 (neu) promoter: Evidence for multiple mechanisms for transcriptional initiation. Mol. Cell. Biol. 7: 2597-2601, 1987. PubMed: 3039351

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