ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Freeze dried E. coli containing the plasmid
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.5; BamHI--4.1, 0.4; HindIII--4.3; PstI--3.1, 1.0, +2 smaller; SalI--2.7, 1.5; SacI--2.65, 1.5.
This is the 5' EcoRI fragment of lambdaHER2-436 which begins about 150 bp upstream of the initiation codon and encodes the first 433 amino acids--including the signal sequence and 4 potential glycosylation sites.
References
Coussens L, et al. Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene. Science 230: 1132-1139, 1985. PubMed: 2999974
Tal M, et al. Human HER2 (neu) promoter: Evidence for multiple mechanisms for transcriptional initiation. Mol. Cell. Biol. 7: 2597-2601, 1987. PubMed: 3039351
