| 產(chǎn)品名稱 |
M-2E6 |
| 商品貨號(hào) |
B187668 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with the Fab portion of human IgM.
|
| Derivation |
Animals were immunized with purified human IgM. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human IgM |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human IgM |
| Comments |
The antibody reacts with the Fab portion of human IgM.
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Isotype |
IgG1 |
| Name of Depositor |
B Forghani, NJ Schmidt |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Forghani B, et al. Use of monoclonal antibodies to human immunoglobulin M in "capture" assays for measles and rubella immunoglobulin M. J. Clin. Microbiol. 18: 652-657, 1983. PubMed: 6355153
Forghani B, et al. Production of monoclonal antibodies to human IgM for assay of viral IgM antibodies. J. Virol. Methods 5: 317-327, 1982. PubMed: 6819305
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