| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--22.3, 16.0, 9.2, 2.6, 2.0; EcoRI/HindIII--22.3, 16.0, 9.2, 2.6, 2.0. Verified to contain the correct insert sequence using the following oligonucleotides (5'-3'): CTTCACCACAGTCTGACAACTACC and TTTTCCAGTATGTCGTATCTTAGGTC. The predicted product size is (bp): 115. The observed product size is (bp): 115. |