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p1-79
p1-79
規(guī)格:
價格:
編號:B188811
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱
p1-79
商品貨號
B188811
Designations
p1-79
Species
Homo sapiens, human
培養(yǎng)基
Depositors
M Litt
Applications
Studies of 3-generation Utah families suggest the numerous fragments homologous to this probe are inherited as haplotypes.
Can use the entire plasmid as a probe.
Vector
Construct size (kb): 3.799999952316284
Insert
DNA: genomic
Insert lengths(kb): 0.8999999761581421
Gene product: DNA Segment, repetitive [D1Z2]
Insert Size (kb)
0.900
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.1, 0.64; PstI--3.7; EcoRI--3.2, 0.64; HindIII--3.8; SmaI--3.8.
The same locus was independently ascertained by Y. Nakamura.
Studies of 3-generation Utah families suggest the numerous fragments homologous to this probe are inherited as haplotypes.
This probe is a subclone of cosmid 2-30 from a human genomic library.
Insert contains several copies of a 39 bp repeat. Variation in copy number may be basis for multiple alleles.
TaqI reveals at least 50 fragments (from <1 to >50 kb), many of which are polymorphic. Each of 27 unrelated individuals tested showed distinct band pattern. Similar variation seen with HaeIII, MspI, PstI and PvuII.
Can use the entire plasmid as a probe. Hybridizes with unusually high efficiency.
References

Buroker N, et al. A hypervariable repeated sequence on human chromosome 1p36. Hum. Genet. 77: 175-181, 1987. PubMed: 2888721

Nakamura Y, et al. Characterization of a human 'midisatellite' sequence. Nucleic Acids Res. 15: 2537-2547, 1987. PubMed: 3031603

Cogen PH, et al. Deletion mapping of the medulloblastoma locus on chromosome 17p. Genomics 8: 279-285, 1990. PubMed: 1979050

Dracopoli NC, et al. The CEPH consortium linkage map of human chromosome 1. Genomics 9: 686-700, 1991. PubMed: 2037294

Michael Litt, personal communication

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