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Suid herpesvirus 1
Suid herpesvirus 1
規格:
貨期:
編號:B188982
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Suid herpesvirus 1
商品貨號
B188982
Classification
Herpesviridae, Varicellovirus
Deposited As
Pseudorabies virus
Agent
Suid herpesvirus 1
Common Name
Pseudorabies virus
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
derived from PRV dl tk (Kit), ATCC VR-2074
Product Format
frozen
Storage Conditions
-70°C or colder
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Comments
Live marker vaccine of PRV attenuated by deletions in thymidine kinase gene. Also has deletion of PRV g92 (gIII) gene.
Cultivate virus at an m.o.i. of 0.01 PFU/ml in rab cell culture at 34.5C. Harvest. Sonicate prior to infecting new cells. Virus replicates at 39C but does not induce thymidine kinase activity or a late glycoprotein due to deletion mutations.
Effect on Host
Cytopathic effects in tissue culture
Recommended Host
rabbit cells
swine testicle cells; RAB-9 cells (ATCC ); cultures of bovine, murine, canine, feline or porcine cells
Growth Conditions
Incubation:: 3 days at 30°C or 39°C
Name of Depositor
NovaGene, Inc., Baylor College of Medicine
U.S. Patent
U.S. Patent Number
Source
derived from PRV dl tk (Kit), ATCC VR-2074
References

Kit S, et al. Second-generation pseudorabies virus vaccine with deletions in thymidine kinase and glycoprotein genes. Am. J. Vet. Res. 48: 780-793, 1987. PubMed: 3035972

Kit M, Kit S. Pseudorabies virus mutants, vaccines containing same, methods for the production of same and methods for the use of same. US Patent 4,711,850 dated Dec 8 1987

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