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pFa6a-KANMX6-pADH1-GST
pFa6a-KANMX6-pADH1-GST
規(guī)格:
價(jià)格:詢(xún)價(jià)
編號(hào):B189614
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
pFa6a-KANMX6-pADH1-GST
商品貨號(hào)
B189614
Designations
pFa6a-KANMX6-pADH1-GST
Depositors
DJ DeMarini
培養(yǎng)基
Biosafety Level
1
Vector Information
Vector: pFa6a-KANMX6-pADH1-GST (plasmid)
Construction: pCRTOPO2.1
Marker(s):ampR
Construct size (kb): 5.557000160217285
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): BglII--5.6; EcoRI--5.6; BglII/EcoRI--4.4, 1.3.
The marker, promoter and GST cassette is flanked by the F4 primer site (5') and the R4 primer site (3'). The size of the F4/R4 PCR product is 2692 nt.
F4 primer sequence: 5'-(gene-specific sequence)GAATTCGAGCTCGTTTAAAC-3'. R6 primer sequence: 5'-(gene-specific sequence) CATTGTATATGAGATAGTTGA-3'.
R4 primer sequence: 5'-(gene-specific sequence) ACGCGGAACCAGATCCGATT-3'. The gene-specific sequences of these primers are chosen to correspond to the reverse complement of the N-terminal codons of the target gene.
Contains a functional truncated (700 nt) ADH promoter. The promoter is not down regulated during the exponential phase.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

DeMarini DJ, et al. Constitutive promoter modules for PCR-based gene modification in Saccharomyces cerevisiae. Yeast 18: 723-728, 2001. PubMed: 11378899

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component of:ATCC 87819
Shipped
freeze-dried
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