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pMWNT3A plasmid in E. coli GC10
pMWNT3A plasmid in E. coli GC10
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B189727
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pMWNT3A plasmid in E. coli GC10
商品貨號(hào)
B189727
Designations
pMWNT3A plasmid in E. coli GC10
GenBank Definition

Mutated version- (tryptophan 539 to leucine)

培養(yǎng)基
GenBank Number

X56842

Species
Mus musculus, mouse
Depositors
Roel Nusse
Vector
Construct size (kb): 4.65
Insert site: EcoRI
Insert
DNA: cDNA
Insert size: 1.45
Gene: Wingless-type MMTV integration site family member 3A, Wnt-3A
Source: Mus musculus
Putative ID
wingless-related MMTV integration site 3A
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
?Frozen glycerol stock of Escherichia coli GC10 containing the plasmid.
Comments
This is a full length cDNA clone of the mouse Wnt-3A gene cloned into the EcoRI site of the pGEM-3Zf vector.
The insert corresponds to nt 1-1450 of the Wnt-3A sequence in Genbank X56842.
restriction digests of the clone give the following sizes (in kb): SmaI - 3.7, 1.0; EcoRI - 3.2, 1.45; BglI - 2.5, 1.5, 0.7; EcoRI/HindIII - 3.2, 1.45. - ATCC staff
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Roelink H, et al. Molecular cloning and chromosomal localization to 17q21 of the human WNT3 gene. Genomics 17: 790-792, 1993.

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