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HIBAA50
HIBAA50
規格:
價格:
編號:B189827
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HIBAA50
商品貨號
B189827
Designations
HIBAA50
GenBank Number

T08008

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 5.400000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, <-, 100-556
insert detection: lacZ', <-, 594-1119
primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, <-
promoter: lac, <-, 1164-1192
replicon: pMB1, <-, 1518-1518
marker(s): ampR, <-, 2276-3136
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PM5*
Gene name: pM5 protein
Contains complete coding sequence?: N
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 2.1
Cross references: DNA Seq. Acc.: T08008; EST: EST05899; Primer: M13-21; THC
id: THC122871
DNA Seq. Acc.: T08009; EST: EST05900; Primer: M13 Reverse;
THC id: THC122871
DNA Seq. Acc.: X57398; EST: EST05899
Nucleotides 1-221 of the insert correspond to
nucleotides 4068-3848 of X57398 with 91% identity.
DNA Seq. Acc.: X57398; EST: EST05900; Primer: M13 Reverse
Nucleotides 8-336 of the insert correspond to
nucleotides 2058-2385 of X57398 with 97% identity.
Insert lengths(kb): 2.099999904632568
Tissue: infant brain, 3 months old (from Bento Soares)
Gene product: pM5 protein [PM5*]
Insert Size (kb)
2.100
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.0, 1.5; HindIII/NotI--3.4, 1.0, 0.6, 0.54.
Based on the alignment with GenBank X57398 (4086 nt, CDS 1-3573) and the size of the insert, this appears to include part of the coding sequence and the 3' untranslated region.
References

Adams MD, et al. Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library. Nat. Genet. 4: 373-380, 1993. PubMed: 8401585

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