| Vector |
Construct size (kb): 5.400000095367432 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, <-, 100-556
insert detection: lacZ', <-, 594-1119
primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, <-
promoter: lac, <-, 1164-1192
replicon: pMB1, <-, 1518-1518
marker(s): ampR, <-, 2276-3136
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PM5*
Gene name: pM5 protein
Contains complete coding sequence?: N
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 2.1
Cross references: DNA Seq. Acc.: T08008; EST: EST05899; Primer: M13-21; THC
id: THC122871
DNA Seq. Acc.: T08009; EST: EST05900; Primer: M13 Reverse;
THC id: THC122871
DNA Seq. Acc.: X57398; EST: EST05899
Nucleotides 1-221 of the insert correspond to
nucleotides 4068-3848 of X57398 with 91% identity.
DNA Seq. Acc.: X57398; EST: EST05900; Primer: M13 Reverse
Nucleotides 8-336 of the insert correspond to
nucleotides 2058-2385 of X57398 with 97% identity. Insert lengths(kb): 2.099999904632568 Tissue: infant brain, 3 months old (from Bento Soares) Gene product: pM5 protein [PM5*] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--4.0, 1.5; HindIII/NotI--3.4, 1.0, 0.6, 0.54. Based on the alignment with GenBank X57398 (4086 nt, CDS 1-3573) and the size of the insert, this appears to include part of the coding sequence and the 3' untranslated region. |
