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phTGF1-10-925
phTGF1-10-925
規格:
價格:詢價
編號:B189837
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
phTGF1-10-925
商品貨號
B189837
Designations
phTGF1-10-925
GenBank Number

X70340

培養基
Species
Homo sapiens, human
Depositors
GI Bell
Vector
Construct size (kb): 4.300000190734863
Insert
DNA: cDNA
Insert lengths(kb): 0.925000011920929
Tissue: kidney
Gene product: transforming growth factor, alpha(transforming growth factor alpha) [TGFA]
Alleles: A1, A2, B1, B2
Insert Size (kb)
0.925
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
The insert contains the following restriction sites, in bp from the 5' end of the insert: PstI (167), PstI (234), RsaI (285), XmnI (590).
Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 0.9; BglI--1.5, 1.1 (doublet), 0.3; SphI--2.9, 1.1; BamHI--4.3; PvuI--4.3.
The insert includes 49 bp of 5' untranslated, 480 bp encoding transforming growth factor alpha precursor, and 391 bp 3' untranslated.
The RsaI polymorphism is in linkage disequilibrium with a BamHI polymorphism detected by phTGF1-10-3350, (r=0.55, p=0.03).
Enzyme(s) not detecting polymorphism: BclII, BglII, EcoRI, EcoRV, HaeIII, HindIII, HinfI, KpnI, MspI, PstI, PvuII, SacI, XbaI, XhoI.
References

Tricoli JV, et al. The gene for human transforming growth factor alpha is on the short arm of chromosome 2. Cytogenet. Cell Genet. 42: 94-98, 1986. PubMed: 3459638

Murray JC, et al. RFLPs for transforming growth factor alpha (TGFA) gene at 2p13. Nucleic Acids Res. 14: 7136, 1986. PubMed: 2876411

Murray JC, et al. Linkage of an autosomal dominant clefting syndrome (Van der Woude) to loci on chromosome 1q. Am. J. Hum. Genet. 46: 486-491, 1990. PubMed: 2309700

Graeme I Bell, personal communication

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