| 產品名稱 |
pGEM4Z/CREB2 |
| 商品貨號 |
B190394 |
| Designations |
pGEM4Z/CREB2 |
| GenBank Number |
M86842
|
| Species |
Homo sapiens, human |
| Depositors |
JM Leiden |
| Applications |
in another host, produces protein cAMP responsive element binding protein 2 |
| Vector |
Construct size (kb): 5.300000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-4Z
Intact vector size: 3.000
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI
SphI HindIII
Polylinker sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI
SphI HindIII
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CREB2
Gene name: cAMP responsive element binding protein 2
Contains complete coding sequence?: U
Chromosome: 2; Localization: 2 q32
Tissue: acute T cell leukemia, Jurkat cell line
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.3
Cross references: DNA Seq. Acc.: M86842 Insert lengths(kb): 1.299999952316284 Tissue: acute T cell leukemia, Jurkat cell line Gene product: cAMP responsive element binding protein 2 [CREB2] Target Gene: cAMP responsive element binding protein 2 |
| Insert Size (kb) |
1.300 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 1.40; KpnI--4.2, 1.05; PstI--5.3; BamHI--5.3; EcoRV--3.8, 0.72, 0.45. The insert contains the following restriction sites (nt from the 5' EcoRI site): XmnI--383; EcoRV--667; KpnI--991. Detects a transcript of 1.5 kb in human cells. Has also been used to detect mouse transcripts. |
| References |
Karpinski BA, et al. Molecular cloning of human CREB-2: an ATF/CREB transcription factor that can negatively regulate transcription from the cAMP response element. Proc. Natl. Acad. Sci. USA 89: 4820-4824, 1992. PubMed: 1534408
Jeffrey M Leiden, personal communication
|
