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pGEM4Z/CREB2
pGEM4Z/CREB2
規格:
價格:詢價
編號:B190394
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pGEM4Z/CREB2
商品貨號
B190394
Designations
pGEM4Z/CREB2
GenBank Number

M86842

培養基
Species
Homo sapiens, human
Depositors
JM Leiden
Applications
in another host, produces protein cAMP responsive element binding protein 2
Vector
Construct size (kb): 5.300000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-4Z
Intact vector size: 3.000
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI
SphI HindIII
Polylinker sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI
SphI HindIII
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CREB2
Gene name: cAMP responsive element binding protein 2
Contains complete coding sequence?: U
Chromosome: 2; Localization: 2 q32
Tissue: acute T cell leukemia, Jurkat cell line
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.3
Cross references: DNA Seq. Acc.: M86842
Insert lengths(kb): 1.299999952316284
Tissue: acute T cell leukemia, Jurkat cell line
Gene product: cAMP responsive element binding protein 2 [CREB2]
Target Gene: cAMP responsive element binding protein 2
Insert Size (kb)
1.300
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 1.40; KpnI--4.2, 1.05; PstI--5.3; BamHI--5.3; EcoRV--3.8, 0.72, 0.45.
The insert contains the following restriction sites (nt from the 5' EcoRI site): XmnI--383; EcoRV--667; KpnI--991.
Detects a transcript of 1.5 kb in human cells. Has also been used to detect mouse transcripts.
References

Karpinski BA, et al. Molecular cloning of human CREB-2: an ATF/CREB transcription factor that can negatively regulate transcription from the cAMP response element. Proc. Natl. Acad. Sci. USA 89: 4820-4824, 1992. PubMed: 1534408

Jeffrey M Leiden, personal communication

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