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cos15
cos15
規格:
價格:
編號:B190602
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
cos15
商品貨號
B190602
Designations
cos15
GenBank Number

U07563

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 37.82300186157227
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: svPHEP
Intact vector size: 9.201
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI ClaI
Polylinker sites: HindIII ClaI EcoRI BamHI
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
replicon: pMB1
Cross references: DNA Seq. Acc.: L19900
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ABL1
Other gene symbols: ABL
Genomic copy number: unique
Gene name: Abelson murine leukemia viral (v-abl) oncogene homolog 1
Contains complete coding sequence?: N
Chromosome: 9; Localization: 9 q34.1
Tissue: lung carcinoma
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb): 28.622
Cross references: DNA Seq. Acc.: U07563
Nucleotides 1-29622 of the insert correspond to
nucleotides 44062-73683 of U07563.
Insert lengths(kb): 28.62199974060059
Tissue: lung carcinoma
Gene product: Abelson murine leukemia viral (v-abl) oncogene homolog 1 [ABL]
Alleles: D1, D2, D1, D2, A1, A2, B2, C1, C2, A1, A2, B1, B2, C1, C2, B1
Insert Size (kb)
28.622
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--15.5, 12.5, 7.7, 3.6, 0.65; BglII--8.0, 7.7, 7.0, 4.6, 4.4, 3.1, 2.55, 2.3, 1.75, 1.5, 0.68; EcoRI--7.7 (doublet), 6.2, 3.4 (doublet), 3.1, 2.65, 2.3, 1.85, 1.15, 0.84;
HindIII--9.2, 7.7, 5.7, 4.6, 3.3, 3.0, 2.65, 1.8, 1.3, 0.98; NotI-->23.0; SstI--8.0, 7.7, 4.1 (doublet), 3.4, 3.1, 2.4, 2.3, 2.0, 1.55, 1.15.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
The insert begins in intron 1a and extends into intron 8.
References

Groffen J, et al. Isolation of human oncogene sequences (v-fes homolog) from a cosmid library. Science 216: 1136-1138, 1982. PubMed: 6281890

Heisterkamp N, et al. The human v-abl cellular homologue. J. Mol. Appl. Genet. 2: 57-68, 1983. PubMed: 6302194

Chissoe SL, et al. Sequence and analysis of the human ABL gene, the BCR gene, and regions involved in the Philadelphia chromosomal translocation. Genomics 27: 67-82, 1995. PubMed: 7665185

Bruce A Roe, personal communication

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