Restriction digests of the clone give the following sizes (kb): BamHI/XbaI--6.0, 4.5, 2.8; SalI--13.5; SmaI--13.5.
LEU2-d is a promoter-defective allele of the yeast LEU2 gene, which increases plasmid copy number to 200-300 copies in the presence of LEU2 selection (host grown in medium lacking leucine).
YE-type shuttle vector.
The order of the major features in this plasmid is: LEU2-d - ori(2 micron) - BamHI site - SalI site - URA3 - ampR.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Ma H, et al. Plasmid construction by homologous recombination in yeast. Gene 58: 201-216, 1987. PubMed: 2828185
Rose AB, Broach JR. Propagation and expression of cloned genes in yeast: 2-micron circle-based vectors. Methods Enzymol. 185: 234-279, 1990. PubMed: 2199781
