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pUC1318
pUC1318
規格:
價格:
編號:B190786
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pUC1318
商品貨號
B190786
Designations
pUC1318
Depositors
R Kay
培養基
Biosafety Level
1
Vector Information
Size (kb): 2.7300000190734860
Vector: pUC1318 (plasmid)
Promoters: Promoter lac
Construction: pUC13, pUC18
Marker(s):ampR
Construct size (kb): 2.730000019073486
Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.50; HindIII--2.55; KpnI--2.55.
A pUC type plasmid vector with polylinker designed for addition of new restriction sites to the ends of DNA fragments.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470

Kay R, McPherson J. Hybrid pUC vectors for addition of new restriction enzyme sites to the ends of DNA fragments. Nucleic Acids Res. 15: 2778, 1987. PubMed: 3031611

Shipped
freeze-dried
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