Restriction digests of the clone give the following sizes (kb): EcoRI--2.50; HindIII--2.55; KpnI--2.55.
A pUC type plasmid vector with polylinker designed for addition of new restriction sites to the ends of DNA fragments.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470
Kay R, McPherson J. Hybrid pUC vectors for addition of new restriction enzyme sites to the ends of DNA fragments. Nucleic Acids Res. 15: 2778, 1987. PubMed: 3031611
