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Size (kb): 42.6

DESCRIPTION OF VECTOR:
Intact vector size: 42.600
Type of vector: phage
Cloning sites: XhoI, EcoRI
Polylinker sites: EcoRI XhoI EcoRI
Construction: lambdagt6; pSE936
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
other: lox, ->
replicon: ARS1
replicon: pMB1
marker(s): ampR, <-
promoter for expression: GAL1, ->
MCS: XhoI EcoRI, ->
promoter for expression: lac, <-
transcription terminator: HIS3, ->
marker(s): URA3, ->
centromere: CEN4
other: lox, ->
other: right arm (15.2 kb, with cI857)

Vector: lambdaYES-R (phage, lambda - replacement)

Promoters: Promoter for expression GAL1

Construction: lambdagt6; pSE936

Marker(s):URA3,ampR

Construct size (kb): 42.6

Features: marker(s): URA3
marker(s): ampR
other: left arm (19.6 kb)
other: lox
other: right arm (15.2 kb, with cI857)
promoter for expression: GAL1
promoter for expression: lac
replicon: ARS1
replicon: pMB1
MCS: XhoI EcoRI
transcription terminator: HIS3
centromere: CEN4

expression vector

lambda vector allowing conversion to plasmid

vector for constructing cDNA libraries

Restriction digests of the clone give the following sizes (kb): EcoRI--24.0, 20.0; NotI--44.0; XhoI--24.0, 20.0.

The plasmid derivative (pSE936, 7.8 kb) is a YC-type shuttle expression vector.

Transcription initiated from the GAL1 promoter in yeast hosts can be terminated at the HIS3 terminators. GAL1 does not provide translational start signals.

Vector for the construction of cDNA libraries permitting regulated expression in both yeast and E. coli and simple conversion to plasmid via the cre-lox system.

The lac promoter contains a ribosome binding sequence optimally spaced from an AUG start codon and can support translational fusions.

Temperature: 37.0°C