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YIp28 [pRB21]
YIp28 [pRB21]
規(guī)格:
價(jià)格:
編號(hào):B191795
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
YIp28 [pRB21]
商品貨號(hào)
B191795
Designations
YIp28 [pRB21]
Depositors
D Botstein
培養(yǎng)基
Biosafety Level
1
Vector Information
Size (kb): 7.8000001907348630
Vector: YIp28 (plasmid)
Construction: pBR322, Saccharomyces cerevisiae
Marker(s):LEU2,URA3,ampR
Construct size (kb): 7.800000190734863
Features: marker(s): ampR, LEU2, URA3
replicon: pMB1
Applications
YI-type (integrating) shuttle vector
integrating vector
shuttle vector
Comments
A general purpose integrating Saccharomyces cerevisiae/Escherichia coli shuttle vector.
Orientations are such that transcription from URA3 is clockwise and from LEU2 is counterclockwise.
A 1.1 kb HindIII fragment containing URA3 and a 2.2 kb SalI/XhoI fragment containing LEU2 were cloned into the HindIII and SalI sites, respectively, of pBR322. Unique restriction sites are in the 3' untranslated regions of URA3(SmaI) and LEU2(SalI).
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Shipped
freeze-dried
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